This course deals with the advanced technical principles involved in the analysis of gene expression: reporter genes, antisense RNA and differential cDNA libraries. Techniques for in-vivo and in-vitro production of proteins (fusion proteins and the two-hybrid system) are discussed. Finally, advanced applications of the polymarase chain reaction (PCR), including real-time PCR, are reviewed. PREREQ: CHMI 3226.(lec 3) cr 3
